Chemical Characterization of Salvia officinalis (Sage) Extract and Preliminary Antibacterial Assessment Against Staphylococcus epidermidis

Maryam Latif Abdullah1, Latif Abdullah Hamad1
1College of Science, Al-Farabi University, Iraq
DOI: https://doi.org/10.70517/revcc2624171
Published: 15/10/2026
: Maryam Latif Abdullah, Latif Abdullah Hamad. Chemical Characterization of Salvia officinalis (Sage) Extract and Preliminary Antibacterial Assessment Against Staphylococcus epidermidis. Revista Cultura Científica, 2026 Issue 24. pg. 2058-2092.

Abstract

Salvia officinalis L. (common sage) is an aromatic medicinal plant whose extract composition and potential antibacterial properties depend on the preparation and testing conditions. This descriptive laboratory study reports elemental measurements, infrared spectral observations, gas chromatography–mass spectrometry (GC–MS) output and a preliminary agar-diffusion assessment against a bacterium identified in the source manuscript as Staphylococcus epidermidis. The reported elemental concentrations were nitrogen 1.30%, phosphorus 0.50%, potassium 1.68%, calcium 0.126% and sulfur 0.0591%, equivalent to 13,000, 5,000, 16,800, 1,260 and 591 parts per million, respectively, on the same stated percentage basis. An infrared spectrum and GC–MS chromatographic and library-search images were supplied, providing preliminary evidence of chemically heterogeneous extract material but not a validated quantitative identification of individual bioactive compounds. The antimicrobial plate supplied with the study showed no clearly discernible inhibition halos around the tested preparations. This observation should be understood as an absence of visible agar-diffusion inhibition under the photographed conditions, not proof that the plant lacks antibacterial activity or clinical efficacy. Important reporting limitations include inconsistent solvent names, incomplete units for the stated concentration series, an unsubstantiated tabulated p-value, and absent details of bacterial strain verification, assay replication, instrument settings and chromatographic identification thresholds. The findings therefore constitute an initial analytical and microbiological description requiring standardized replication before firm chemical, antibacterial or therapeutic conclusions can be drawn.

Keywords:

Salvia officinalis; sage; Staphylococcus epidermidis; elemental analysis; FTIR; GC–MS; agar diffusion

Abstract

Salvia officinalis L. (common sage) is an aromatic medicinal plant whose extract composition and potential antibacterial properties depend on the preparation and testing conditions. This descriptive laboratory study reports elemental measurements, infrared spectral observations, gas chromatography–mass spectrometry (GC–MS) output and a preliminary agar-diffusion assessment against a bacterium identified in the source manuscript as Staphylococcus epidermidis. The reported elemental concentrations were nitrogen 1.30%, phosphorus 0.50%, potassium 1.68%, calcium 0.126% and sulfur 0.0591%, equivalent to 13,000, 5,000, 16,800, 1,260 and 591 parts per million, respectively, on the same stated percentage basis. An infrared spectrum and GC–MS chromatographic and library-search images were supplied, providing preliminary evidence of chemically heterogeneous extract material but not a validated quantitative identification of individual bioactive compounds. The antimicrobial plate supplied with the study showed no clearly discernible inhibition halos around the tested preparations. This observation should be understood as an absence of visible agar-diffusion inhibition under the photographed conditions, not proof that the plant lacks antibacterial activity or clinical efficacy. Important reporting limitations include inconsistent solvent names, incomplete units for the stated concentration series, an unsubstantiated tabulated p-value, and absent details of bacterial strain verification, assay replication, instrument settings and chromatographic identification thresholds. The findings therefore constitute an initial analytical and microbiological description requiring standardized replication before firm chemical, antibacterial or therapeutic conclusions can be drawn.

Keywords:

Salvia officinalis; sage; Staphylococcus epidermidis; elemental analysis; FTIR; GC–MS; agar diffusion

References

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  2. Khalil, R., and Li, Z.-G. (2011). Antimicrobial activity of essential oil of Salvia officinalis L. collected in Syria. African Journal of Biotechnology, 10(42), 8397–8402. https://doi.org/10.5897/AJB10.2615.

  3. Rădulescu, V., Chiliment, S., and Oprea, E. (2004). Capillary gas chromatography–mass spectrometry of volatile and semi-volatile compounds of Salvia officinalis. Journal of Chromatography A, 1027(1–2), 121–126. https://doi.org/10.1016/j.chroma.2003.11.046.

  4. Horiuchi, K., Shiota, S., Hatano, T., Yoshida, T., Kuroda, T., and Tsuchiya, T. (2007). Antimicrobial activity of oleanolic acid from Salvia officinalis and related compounds on vancomycin-resistant enterococci (VRE). Biological & Pharmaceutical Bulletin, 30(6), 1147–1149. https://doi.org/10.1248/bpb.30.1147.

  5. Mokhtari, R., Kazemi Fard, M., Rezaei, M., Moftakharzadeh, S. A., and Mohseni, A. (2023). Antioxidant, antimicrobial activities, and characterization of phenolic compounds of thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and thyme–sage mixture extracts. Journal of Food Quality, 2023, Article 2602454. https://doi.org/10.1155/2023/2602454.

  6. Cheung, G. Y. C., and Otto, M. (2025). Staphylococcus epidermidis—key to understanding biofilms, commensalism, and more. Journal of Bacteriology, 207(9), e00165-25. https://doi.org/10.1128/jb.00165-25.

  7. Clinical and Laboratory Standards Institute (CLSI). (2024). Performance standards for antimicrobial disk susceptibility tests (14th ed., CLSI standard M02). CLSI. https://clsi.org/shop/standards/m02/.

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Maryam Latif Abdullah
College of Science, Al-Farabi University, Iraq
Latif Abdullah Hamad
College of Science, Al-Farabi University, Iraq

How to cite:

Maryam Latif Abdullah, Latif Abdullah Hamad. Chemical Characterization of Salvia officinalis (Sage) Extract and Preliminary Antibacterial Assessment Against Staphylococcus epidermidis. Revista Cultura Científica, 2026 Issue 24. pg. 2058-2092.

Publication History

Copyright © 2026, Maryam Latif Abdullah, Latif Abdullah Hamad. Published by Revista Cultura Científica. This article is published as open access under the Creative Commons Attribution 4.0 International (CC BY 4.0) license (http://creativecommons.org/licenses/by/4.0/).

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