Effect of Planting Spacing and Some Growth Regulators on Growth, Yield and Antioxidant Activity of Flax

Zahraa Abdel Karim Mazhar1, Rafid Saleh Nahhab2, Maher H. S. Al-Mohammad1
1Department of Field Crops, College of Agriculture, Al-Qasim Green University, Babylon, Iraq
2Department Horticulture & Landscape, College of Agriculture, Al-Qasim Green University, Babylon, Iraq
Published: 18/06/2026
: Zahraa Abdel Karim Mazhar, Rafid Saleh Nahhab, Maher H. S. Al-Mohammad. Effect of Planting Spacing and Some Growth Regulators on Growth, Yield and Antioxidant Activity of Flax. Cultura Científica, 2026 Issue 24. pg. 638-646.

Abstract

An experimental study has been carried out in the 2024 growing season for the impact of planting distances and some growth regulators on the growth, productivity and antioxidant activity of the flax plant. The factors investigated included: Planting distance between roots (5 cm and 15 cm), spraying with growth regulators (distilled water, IAA with a concentration of 2 mg L-1, GA with a concentration of 200 mg L-1, and its combination). The results indicated the superiority of the 15 cm distance in terms of maximum plant height (87.25 cm), number of branches (13.70 branches per plant), stem diameter (1.9667 mm), number of leaves (124.85 leaves per plant), leaf area (0.9303 cm2), total chlorophyll content (5.261 mg 100 g-1 fresh weight), number of capsules (36.72 plant capsules-1), 1000 seeds weight (8.556 g), total seed yield (2.669 tons ha-1), oil percent (34.944%), total oil yield (94.4 kg ha-1), total phenols (15.17 mg g-1 dry weight), total flavonoids (9.55 mg g-1 dry weight), total carotenoids (2.784 mg g-1), total antioxidant capacity (62.44 mg g-1 dry weight), and free radical scavenging efficiency (66.07%). The combined treatment of IAA and GA3 also proved to be superior by obtaining the highest number of branches (19.42 branches plant-1), number of leaves (154.92 leaves plant-1), total chlorophyll content (5.332 mg g-1 dry weight), number of capsules (46.06 capsules plant-1), weight of 1000 seeds (9.790 g), total seed yield (3.548 tons ha-1), oil percent (35.283%), total oil yield (125.4 kg ha-1), total phenols (16.06 mg g-1 dry weight), total flavonoids (10.25 mg g-1 dry weight), total carotenoids (2.960 mg g-1 dry weight). The total antioxidant capacity was 63.45 mg g-1 dry weight and the free radical scavenging efficiency was 67.58%. The treatment P15×IAA2+GA200 had the highest number of branches (19.94 branches-1), number of leaves (159.80 leaves-1), number of capsules (49.05 capsules-1), weight of 1000 seeds (10.407 g), total seed yield (3.822 tons ha-1), percentage of oil (36.090%), total oil yield (137.9 kg ha-1), total phenolic content (17.24 mg g ha-1.9 kg ha-1), total phenolics content (17.24 mg g-1 dry weight), total flavonoids (11.33 mg g-1 dry weight), total carotenoids (3.241 mg g-1 dry weight), total antioxidant capacity (64.30 mg g-1 dry weight) and free radical scavenging efficiency (68.80%).

Keywords: Flax, planting distances, auxin (IAA), gibberellin (GA\(_3\))

Resumen

An experimental study has been carried out in the 2024 growing season for the impact of planting distances and some growth regulators on the growth, productivity and antioxidant activity of the flax plant. The factors investigated included: Planting distance between roots (5 cm and 15 cm), spraying with growth regulators (distilled water, IAA with a concentration of 2 mg L-1, GA with a concentration of 200 mg L-1, and its combination). The results indicated the superiority of the 15 cm distance in terms of maximum plant height (87.25 cm), number of branches (13.70 branches per plant), stem diameter (1.9667 mm), number of leaves (124.85 leaves per plant), leaf area (0.9303 cm2), total chlorophyll content (5.261 mg 100 g-1 fresh weight), number of capsules (36.72 plant capsules-1), 1000 seeds weight (8.556 g), total seed yield (2.669 tons ha-1), oil percent (34.944%), total oil yield (94.4 kg ha-1), total phenols (15.17 mg g-1 dry weight), total flavonoids (9.55 mg g-1 dry weight), total carotenoids (2.784 mg g-1), total antioxidant capacity (62.44 mg g-1 dry weight), and free radical scavenging efficiency (66.07%). The combined treatment of IAA and GA3 also proved to be superior by obtaining the highest number of branches (19.42 branches plant-1), number of leaves (154.92 leaves plant-1), total chlorophyll content (5.332 mg g-1 dry weight), number of capsules (46.06 capsules plant-1), weight of 1000 seeds (9.790 g), total seed yield (3.548 tons ha-1), oil percent (35.283%), total oil yield (125.4 kg ha-1), total phenols (16.06 mg g-1 dry weight), total flavonoids (10.25 mg g-1 dry weight), total carotenoids (2.960 mg g-1 dry weight). The total antioxidant capacity was 63.45 mg g-1 dry weight and the free radical scavenging efficiency was 67.58%. The treatment P15×IAA2+GA200 had the highest number of branches (19.94 branches-1), number of leaves (159.80 leaves-1), number of capsules (49.05 capsules-1), weight of 1000 seeds (10.407 g), total seed yield (3.822 tons ha-1), percentage of oil (36.090%), total oil yield (137.9 kg ha-1), total phenolic content (17.24 mg g ha-1.9 kg ha-1), total phenolics content (17.24 mg g-1 dry weight), total flavonoids (11.33 mg g-1 dry weight), total carotenoids (3.241 mg g-1 dry weight), total antioxidant capacity (64.30 mg g-1 dry weight) and free radical scavenging efficiency (68.80%).

Palabras clave: Flax, planting distances, auxin (IAA), gibberellin (GA\(_3\))
Zahraa Abdel Karim Mazhar
Department of Field Crops, College of Agriculture, Al-Qasim Green University, Babylon, Iraq
Rafid Saleh Nahhab
Department Horticulture & Landscape, College of Agriculture, Al-Qasim Green University, Babylon, Iraq
Maher H. S. Al-Mohammad
Department of Field Crops, College of Agriculture, Al-Qasim Green University, Babylon, Iraq

How to cite:

Zahraa Abdel Karim Mazhar, Rafid Saleh Nahhab, Maher H. S. Al-Mohammad. Effect of Planting Spacing and Some Growth Regulators on Growth, Yield and Antioxidant Activity of Flax. Cultura Científica, 2026 Issue 24. pg. 638-646.

Publication History

Copyright © 2026, Zahraa Abdel Karim Mazhar, Rafid Saleh Nahhab, Maher H. S. Al-Mohammad. Published by Cultura Científica. This article is published as open access under the Creative Commons Attribution 4.0 International (CC BY 4.0) license (http://creativecommons.org/licenses/by/4.0/).

Browse Advance Search